Journal: Journal of Clinical Immunology

Article Title: IL-2 Immunotherapy to Recently HIV-1 Infected Adults Maintains the Numbers of IL-17 Expressing CD4+ T (T H 17) Cells in the Periphery

doi: 10.1007/s10875-010-9432-3

Figure Lengend Snippet: Detection of IL-17 secretion and Flow gating strategy to identify CD4+ and CD8+ Cytokine T cell responses. a Elipsot assay for TLR stimulation HIV-1 uninfected ( n = 4) and 2 HIV-1infected subjects on IL-2 and 2 subjects without IL-2 ( n = 4). b Gating strategy for identification of cytokine elicited T cell responses and of T regulatory cell subsets. a−d Plots show PBMCs from a representative HIV-1 infected subject. (See Table ). a−c Intracellular detection of IL-17, IFN-γ, TNF-α, and IL-2 cytokine production elicited by either anti-CD3/CD28 mAb, Gag, or Pp65 peptide pool stimulation by b CD4+ and c CD8+ T cell subsets. Arrowheads indicated the gated population subsequently analyzed. Fluorescence minus one (FMO) samples were used to define the gates used

Article Snippet: Peptide pools were HIV-1 SF2 GAG (4.8 μg/mL, 127 peptides, SynPep) and CMV pp65 (4.25 μg/mL, 138 peptides, SynPep).

Techniques: Infection, Fluorescence

Journal: Journal of Clinical Immunology

Article Title: IL-2 Immunotherapy to Recently HIV-1 Infected Adults Maintains the Numbers of IL-17 Expressing CD4+ T (T H 17) Cells in the Periphery

doi: 10.1007/s10875-010-9432-3

Figure Lengend Snippet: Decline in counts of Gag responsive T cells for ART+IL-2 and no change in pp65 responding. Data shown is the difference between counts of a HIV-1 Gag and b CMV pp65, T cell responses between pre and post IL-2 treatment (visit 2 counts minus visit 1 counts). On the left-hand side of Fig. are changes in counts of CD4+ T cell responses to Gag or CMV peptide stimulation, and on the right-hand side are CD8+ T cell responses to Gag or CMV peptide stimulation ( asterisks , p < 0.05 by sign rank test (change from baseline)). More detail may be found in supplementary Fig.

Article Snippet: Peptide pools were HIV-1 SF2 GAG (4.8 μg/mL, 127 peptides, SynPep) and CMV pp65 (4.25 μg/mL, 138 peptides, SynPep).

Techniques: